GROUP+1

NAMES: Gerard Falzarano, Caroline Hartman, Brandon Latteri, Jacqueline Beck

__The Effect of Temperature on Enzyme activity__

 * Hypothesis**- The disk in the 60 degrees Celsius peroxide will rise the fastest.

H2O2 Peroxide Catalase Three small beakers Two large beakers Hot Plate Ice cubes small paper disks Thermometer Tweezers
 * Materials**-

**II. The Investigation** What are some factors that could change the rate of the enzyme-catalyzed breakdown of hydrogen peroxide? What might influence the effectiveness of catalase at making the reaction occur? Brainstorm with your group and list your ideas below.


 * amount of time the paper is left in the catalase (soak time)
 * size of the paper (surface area)
 * simple technique
 * temperature
 * ph
 * delusion

**III. Data Analysis and Conclusions**

An enzyme is a protein catalyst that acts by lowering activation energy. They consist of one or more polypeptide chains and are named for the substrate. All living things control their reaction with enzymes. For example, when you bite into a cracker, enzymes immediately reacts on the cracker to digest it. The enzyme is this lab is the catalose. The reaction is forming the bubbles to make the paper disk rise to the top.

Temperature: Room (25 degrees celsius) Temperature: Hot (60 degrees celsius) Temperature: Cold (5 degrees celsius)
 * DATA**
 * || 1st trial || 2nd trial || 3rd trial ||
 * time in seconds || 7.56 || 8.75 || 8.13 ||
 * || 1st trial || 2nd trial || 3rd trial ||
 * time in seconds || 1.3 || 2.22 || 2.25 ||
 * || 1st trial || 2nd trial || 3rd trial ||
 * time in seconds || 4.61 || 5.25 || 9.14 ||

- **//Explaining the effect of your variable on your experiment.//**
 * || Trial 1 || Trial 2 || Trial 3 ||  ||   ||
 * 5 || 4.61 || 5.25 || 9.14 ||  ||   ||
 * 25 || 7.56 || 8.75 || 8.13 ||  ||   ||
 * 60 || 1.3 || 2.22 || 2.25 ||  ||   ||
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** Enzymes are affected by high and low temperatures. Enzymes work best at about room temperature. When temperature is raised or lowered, the chances of them working go down. Enzymes work better in temperatures from 20 degrees celsius to about 30 degrees celsius. A change of 1 or 2 degrees can change the effectiveness of the enzymes by 10 or 20 percent. Most enzymes are denatured at about 40 degrees celsius. ** **//- How do your experimental findings compare w//** **//ith accepted scientific knowledge of factors affecting enzyme activity? Explain. 6 pts.//**

=
Scientific research proves that large temperature differences in comparison to an enzymes average temperature in its natural surroundings can lead to a decrease in the speed it takes to break or bond the substrate. This is caused because temperature changes can affect the shape of the enzyme. Since enzymes are usually at human temperature (98.6), our results weren't as exact as they could have been. We found that an increase in temperature leads to faster breaking or bonding of the substrate, which doesnt quite add up with most other scientists results.====== **//- What improvements would you make your experimental design if you had more time and materials//** One way to improve the experimental design is to vary the number of trials made. Instead of three experiments, there could be five. Another way is to used better equipment such as a bunsen burner and different flasks. If another group did the same experiment, we could compare the results.