GROUP+5

NAMES: Rachel Kearney, Shelby Freedman, Lucia Garabo, Casey Rodenberg, Sam Weglin

__ Exploration of the How the Temperature of the Catalase Affects Enzyme Reactivity __ Hypothesis- If the temperature affects the catalase, then the filter paper will rise at opposite times.

Materials- 2 large beakers Ice 3 thermometers Hot plate Beakers with chemicals in it Catalose Small paper disks Tweezers Peroxcide

**II. The Investigation -** 1. Some factors that could change the rate of the enzyme-catalyzed breakdown of hydrogen peroxide would be temperature, the amount of time the paper dots were soaking, and how much catalose the dots were soaking in.

**III. Data Analysis and Conclusions**
 * || 1st Trial || 2nd Trial || 3rd Trial ||
 * Hot (70 degrees Celsius) || 60 seconds || 120 seconds || did not rise ||
 * Cold (8 degrees Celsius) || 5.9 seconds || 4.97 secodns || 7.91 seconds ||
 * Room Temperature (23 degrees Celsius) || 6.5 secodns || 5.09 seconds || 7.03 seconds ||
 * Time is Showed in Seconds*

Enzymes are mainly proteins, that catalyze (i.e., increase the rates of) chemical reactions. The role of enzymes is that they increase the rate of chemical reactions without themselves being consumed or permanently altered by the reaction. Second, they increase reaction rates without altering the chemical equilibrium between reactions and product. Enzymes speed up the rate of chemical reactions because they lower the energy of activation, the energy that must be supplied in order for molecules to react with one another.

**2 H2O2** **à** **2H2O + O2** * A graph of your findings.



**//- What improvements would you make your experimental design if you had more time and materials?//** Run more trials with all different temperatures to see how much it changes the rate of reaction. Also, by running more tests it eliminates variability and taking average. This shows how fast enzymes operate at and the different rates. This will lead to a more accurate conclusion. If you go onto Pearson online biology textbook chapter 5.5 is on enzymes. If you go onto the online activity page two there is an activity that shows reactions to the temperature. The results were that enzymes grow smaller when the temperature is cold; therefore, it takes longer for the substrate to connect but it still does. But is the substrate is too cold then the substrate won’t connect. When the temperature is hotter like 40 degrees Celsius the substrate won’t connect. When the temperature is room temperature then the enzyme will connect and activate faster. For our experiment our way to test enzymes were to put filter paper in a catalyst and then put it into a cold, room temperature, and heated environment. The room temperature rose fairly quickly. The cold temperature took the same amount of time as the room temperature because it wasn’t that cold because the ice had melted. The heated enzyme though had taken a long time more than a minute for each. The scientific results and our results were similar and the experiment was the right procedure of how they had preformed it.

Set Up